trif (Cell Signaling Technology Inc)
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Trif, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trif/TRIF+Antibody/pmc12796343-266-34-42
Average 95 stars, based on 153 article reviews
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Blocking Assay:Article Title: Mitochondrial Reactive Oxygen Species in TRIF-Dependent Toll-like Receptor 3 Signaling in Bronchial Epithelial Cells against Viral Infection Article Snippet: Protein samples (10 μg) for Western blotting were subjected to electrophoresis on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad Laboratories, Hercules, CA, USA). .. After 30 min of blocking with 5% BSA at room temperature, membranes were incubated overnight at 4 °C using specific primary antibodies against TLR3 (Cat# ab62566; Abcam, Cambridge, MA, USA) 1:500, phospho-TLR3 (Tyr759) (Cat# PA5-64722, Invitrogen), Incubation:Article Title: Mitochondrial Reactive Oxygen Species in TRIF-Dependent Toll-like Receptor 3 Signaling in Bronchial Epithelial Cells against Viral Infection Article Snippet: Protein samples (10 μg) for Western blotting were subjected to electrophoresis on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad Laboratories, Hercules, CA, USA). .. After 30 min of blocking with 5% BSA at room temperature, membranes were incubated overnight at 4 °C using specific primary antibodies against TLR3 (Cat# ab62566; Abcam, Cambridge, MA, USA) 1:500, phospho-TLR3 (Tyr759) (Cat# PA5-64722, Invitrogen), Article Title: Pseudomonas aeruginosa -derived DnaJ functions as a novel immunomodulator inducing IFNβ via CME–SGK1–IRF3 axis in macrophages Article Snippet: .. Membranes were blocked with 5% bovine serum albumin for 2 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against IRF3, p-IRF3 (S386), p-IRF3 (S396), TLR2, TLR3, TLR4, TLR10, Western Blot:Article Title: Cinnamaldehyde inhibits cytokine storms induced by the ORF3a protein of SARS-CoV-2 via ROS-elimination in activated T cells. Article Snippet: The following antibodies were used for flow cytometry: PE-CD4, APC-CD8a, FICT-CD4, Percp-CD8a, PE-CD69, APC-Tim3, Percp-CD45, FICT-CD11b, APC-F4/80, PE-IFN-γ, and APC-IL-4 (BD Biosciences, USA). .. The following antibodies were used for Western blotting: HIF-1α, NFAT, p-Stat1, Stat1, p-ERK, ERK, c-Jun, T-bet, p-P38, p38, p-JNK, JNK, IRF-4, other:Article Title: Urolithins and their phase II conjugates cross the blood-brain barrier and exert a stimulus-dependent anti-inflammatory effect on microglial cells by inhibiting NF-κB nuclear translocation. Article Snippet: Protein levels were determined using the DC colorimetric kit (Bio-Rad, Barcelona, Spain). Article Title: Mitochondrial Reactive Oxygen Species in TRIF-Dependent Toll-like Receptor 3 Signaling in Bronchial Epithelial Cells against Viral Infection. Article Snippet: After extensive wash with PBS, protein bands were detected using a Pierce ECL Western Blotting Substrate (Thermo Scientific), and blots were scanned using ChemiDoc XRS+ imaging systems (Bio-Rad Laboratories). |
