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trif  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc trif
    Trif, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trif/TRIF+Antibody/pmc12796343-266-34-42
    Average 95 stars, based on 153 article reviews
    trif - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Mitochondrial Reactive Oxygen Species in TRIF-Dependent Toll-like Receptor 3 Signaling in Bronchial Epithelial Cells against Viral Infection
    Article Snippet: Protein samples (10 μg) for Western blotting were subjected to electrophoresis on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad Laboratories, Hercules, CA, USA). .. After 30 min of blocking with 5% BSA at room temperature, membranes were incubated overnight at 4 °C using specific primary antibodies against TLR3 (Cat# ab62566; Abcam, Cambridge, MA, USA) 1:500, phospho-TLR3 (Tyr759) (Cat# PA5-64722, Invitrogen), TRIF (Cat# 4596, Cell Signaling Technology, Danvers, MA, USA) 1:1000, NF- κ B p65 (F-6) (Cat# sc-8008, Santa Cruz) 1:2000, phospho-NF- κ B p65 (phospho S536) (Cat# ab86299, Abcam) 1:2000, I κ B-α (C-21) (Cat# sc-371, Santa Cruz) 1:2000, phospho-I κ Bα (Ser32/36) (5A5) (Cat# 9246, Cell Signaling Technology) 1:2000, TNF-α (Cat# 3707, Cell Signaling Technology) 1:1000, and MAVS (Cat# 3993, Cell Signaling) 1:1000 at a specified dilution. .. Following BEAS-2B cells harvesting using RIPA Buffer, protein samples were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).

    Incubation:

    Article Title: Mitochondrial Reactive Oxygen Species in TRIF-Dependent Toll-like Receptor 3 Signaling in Bronchial Epithelial Cells against Viral Infection
    Article Snippet: Protein samples (10 μg) for Western blotting were subjected to electrophoresis on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad Laboratories, Hercules, CA, USA). .. After 30 min of blocking with 5% BSA at room temperature, membranes were incubated overnight at 4 °C using specific primary antibodies against TLR3 (Cat# ab62566; Abcam, Cambridge, MA, USA) 1:500, phospho-TLR3 (Tyr759) (Cat# PA5-64722, Invitrogen), TRIF (Cat# 4596, Cell Signaling Technology, Danvers, MA, USA) 1:1000, NF- κ B p65 (F-6) (Cat# sc-8008, Santa Cruz) 1:2000, phospho-NF- κ B p65 (phospho S536) (Cat# ab86299, Abcam) 1:2000, I κ B-α (C-21) (Cat# sc-371, Santa Cruz) 1:2000, phospho-I κ Bα (Ser32/36) (5A5) (Cat# 9246, Cell Signaling Technology) 1:2000, TNF-α (Cat# 3707, Cell Signaling Technology) 1:1000, and MAVS (Cat# 3993, Cell Signaling) 1:1000 at a specified dilution. .. Following BEAS-2B cells harvesting using RIPA Buffer, protein samples were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).

    Article Title: Pseudomonas aeruginosa -derived DnaJ functions as a novel immunomodulator inducing IFNβ via CME–SGK1–IRF3 axis in macrophages
    Article Snippet: .. Membranes were blocked with 5% bovine serum albumin for 2 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against IRF3, p-IRF3 (S386), p-IRF3 (S396), TLR2, TLR3, TLR4, TLR10, TRIF, AKT, p-AKT (S473), SGK1, p-SGK1, and β-actin (Cell Signaling Technology, Danvers, MA, USA). ..

    Western Blot:

    Article Title: Cinnamaldehyde inhibits cytokine storms induced by the ORF3a protein of SARS-CoV-2 via ROS-elimination in activated T cells.
    Article Snippet: The following antibodies were used for flow cytometry: PE-CD4, APC-CD8a, FICT-CD4, Percp-CD8a, PE-CD69, APC-Tim3, Percp-CD45, FICT-CD11b, APC-F4/80, PE-IFN-γ, and APC-IL-4 (BD Biosciences, USA). .. The following antibodies were used for Western blotting: HIF-1α, NFAT, p-Stat1, Stat1, p-ERK, ERK, c-Jun, T-bet, p-P38, p38, p-JNK, JNK, IRF-4, TRIF, p-AKT, AKT, and GAPDH (CST, USA). ..

    other:


    Article Title: Mitochondrial Reactive Oxygen Species in TRIF-Dependent Toll-like Receptor 3 Signaling in Bronchial Epithelial Cells against Viral Infection.
    Article Snippet: After extensive wash with PBS, protein bands were detected using a Pierce ECL Western Blotting Substrate (Thermo Scientific), and blots were scanned using ChemiDoc XRS+ imaging systems (Bio-Rad Laboratories).



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    Image Search Results


    RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and p65 were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.

    Journal: Frontiers in Pharmacology

    Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

    doi: 10.3389/fphar.2026.1655883

    Figure Lengend Snippet: RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and p65 were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.

    Article Snippet: After transfer, the membranes were blocked with sealing liquid and incubated for 2 h. Following blocking, membranes were incubated with primary antibodies against β-actin (dilution 1:1,000, ab8227, Abcam), ALIX (dilution 1:1,000, ab275377, Abcam), TSG101 (dilution 1:1,000, ab125011, Abcam), CD9 (dilution 1:1,000, ab236630, Abcam), Bax (dilution 1:1,000, ab32503, Abcam), Bcl-2 (dilution 1:1,000, ab32124, Abcam), Cleaved caspase 3 (dilution 1:500, ab32042, Abcam), TLR4 (dilution 1:1,000, TA382568, OriGene), TRIF (dilution 1:1,000, TA382523, OriGene), NF-κB p65 (dilution 1:1,000, TA385159, OriGene), Phospho-NF-κB p-p65 (dilution 1:1,000, TA380848, OriGene), STC1 (dilution 1:5000, ab229477, Abcam) at 4 °C overnight.

    Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, Western Blot

    Schematic diagram illustrated that Naoxintong Capsule protected vascular endothelial cell from injury and dysfunction by downregulating miR-382-5p in circulating exosomes, potentially via targeting STC1 and inhibiting the TLR4/TRIF/NF-κB pathway.

    Journal: Frontiers in Pharmacology

    Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

    doi: 10.3389/fphar.2026.1655883

    Figure Lengend Snippet: Schematic diagram illustrated that Naoxintong Capsule protected vascular endothelial cell from injury and dysfunction by downregulating miR-382-5p in circulating exosomes, potentially via targeting STC1 and inhibiting the TLR4/TRIF/NF-κB pathway.

    Article Snippet: After transfer, the membranes were blocked with sealing liquid and incubated for 2 h. Following blocking, membranes were incubated with primary antibodies against β-actin (dilution 1:1,000, ab8227, Abcam), ALIX (dilution 1:1,000, ab275377, Abcam), TSG101 (dilution 1:1,000, ab125011, Abcam), CD9 (dilution 1:1,000, ab236630, Abcam), Bax (dilution 1:1,000, ab32503, Abcam), Bcl-2 (dilution 1:1,000, ab32124, Abcam), Cleaved caspase 3 (dilution 1:500, ab32042, Abcam), TLR4 (dilution 1:1,000, TA382568, OriGene), TRIF (dilution 1:1,000, TA382523, OriGene), NF-κB p65 (dilution 1:1,000, TA385159, OriGene), Phospho-NF-κB p-p65 (dilution 1:1,000, TA380848, OriGene), STC1 (dilution 1:5000, ab229477, Abcam) at 4 °C overnight.

    Techniques: